IN-VITRO AND IN-VIVO ANTI-ASTHMATIC ACTIVITY OF ETHANOLIC LEAF EXTRACT OF CAESALPINIA PULCHERRIMA

The ethanol extract of Caesalpinia pulcherrima was evaluated for antiasthmatic activity by using in vitro and in vivo models. In vitro models isolated guinea pig ileum preparation was studied to assess smooth muscle relaxant activity of Caesalpinia pulcherrima. The study showed that extract is effective against histamine-induced contraction. In isolated guinea pig ileum preparation extract exhibits maximum relaxant effect against histamine at concentrations. In in vivo antiasthmatic models, histamine induced bronchospasm in guinea pigs was adopted to study the antiasthmatic activity of Caesalpinia pulcherrima (200 & 400mg/kg). The preconvulsion time (PCT), i.e. the time of aerosol exposure to the onset of dyspnoea leading to the appearance of convulsion, was noted, followed by preconvulsion dyspnoea was calculated as basal value. Ethanolic leaf extract of Caesalpinia pulcherrima enhanced the time of preconvulsive dysponea against histamine induced dysponea. The results of these studies indicated that ethanolic leaf extract of Caesalpinia Pulcherrima exhibited antiasthmatic activity against both in vitro and in vivo animal models